Measure Cell Respiration of Peas
Cell respiration refers to the process of converting the chemical energy of organic molecules into a form immediately usable by organisms. Glucose may be oxidized completely if sufficient oxygen is available according to the following equation:
C6H12O6 + 6O2(g) → 6 H2O + 6 CO2(g) + energy
All organisms, including plants and animals, oxidize glucose for energy. Often, this energy is used to convert ADP and phosphate into ATP. Peas undergo cell respiration during germination. Do peas undergo cell respiration before germination? Using your collected data, you will be able to answer the question regarding respiration and non-germinating peas
In this experiment, you will
- Measure gas production.
- Study the effect of temperature on cell respiration.
- Determine whether germinating peas and non-germinating peas respire.
- Compare the rates of cell respiration in germinating and non-germinating peas.
On the Go Direct® CO2 Gas Sensor and Go Direct® O2 Gas Sensor, press the power button once. The LED will blink red. Launch the Graphical Analysis App. Click Sensor Data Collection. Now select GDX-O2 and GDX-CO2 from the list (if you have more than one device, select the serial number corresponding to that on the case). The LED on the connected sensor(s) will now blink Green. Once they are both connected. Click DONE.
Place 25 Germinating peas into the respiration chamber. Please the O2 sensor veritcally into the chamber, this should fit snugly in the Black grommet. Place the CO2 in the neck of the Chamber, this one is horizontal. Wait 4 minutes to the chamber to stabilize.
Change the sample duration to 10 minutes, please collect.
Repeat with non-germanting peas.